Tetramorium Caespitum (Pavement Ant)

December 28, 2025

Sample information
Picture
Entry by: KT
Location
Collection date 09/17/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

The ant is smaller, black in color, social arthropod, found near playground, on pavement
Putative identification Arthropoda Insecta Hymenoptera Formicidae Tetramorium Tetramorium caespitum

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes
  1. Lanes 2 and 3 are the Ant DNA samples. Lane 2 is the ant DNA, lane 3 is the Wolbachia DNA.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The procedure of PCR and gel electrophoresis was conducted carefully and correctly. The controls worked.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Tetramorium caespitum was found to be negative for Wolbachia.
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