DEM-CRA Hymenoptera

November 11, 2025

Sample information
Picture
Photos by: Chaeli A.
Location
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected in a floral garden habitat with an aerial sweep net
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

A recent graph study showed that the order Hymenoptera only had a 7% c chance of being infected with Wolbachia, so I was very confident that my insect was not infected.
Wolbachia 16S sequence
Arthropod COI sequence
TTGATcTGGATAGTAGGAACTTCTTTAAGTGTTTTAATTCGTTTGGAACT TGGTActcctttccgattTATTGGAAATGATCAAATTTATAATAGAATTG TAACTGGACATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATA ATTGGAGGATTTGGTAATTGATTAGTTCCTTTAATATTAGGGTGTCCAGA TATAGCTTATCCGCGATTAAATAATATAAGTTATTGATTATTACCCCCTT CTTTAACTTTATTAATTATAAGAAATTTTATTGGTGATGGAGTTGGAACA GGTTGAACAGTTTATCCTCCTTTATCATCTATTCCATATCACTCTGGTAT ATCTGTTGATTTATCAATTATTTCTTTACATATTGCTGGAATTTCTTCTA TTTTAGGAGCTATTAATTTTATAGTAACTATTGTAAATATACATATTAAA ATTTTAAAATTTGATATTTTACCTCTATTACCTTGATCAATTTTTATTAC AGCTTCATTACTTTTATTATCTTTACCTGTTTTAGCAGGAGCTATTACAA TATTATTATTAGACCGTAATTTGAATACTTCTTTTTTTGATCCTTCTGGT GGGGGTGATCCAATTTTATATCAACATCTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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