DEM-MRH Diptera

Sample information
Picture	Photos by: Mackenzie H.
Location	No map location data available.
Collection date	08/26/2025
Captive / Cultivated?	Wild-caught
Group	Georgia Southern University
Observations	Tools Used: Sweep Nets, beating sheets, Specimen Jar, and gloves. Outcome: I collected my Mosquito in the long grasses using the collection jar to catch it. My partner, Autumn collected a dragonfly using the collection jar.
Putative identification	Arthropoda Insecta
Methods
Extraction kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes	DNA extraction kit of in-house reagents was used.
Results
Wolbachia presence	Unknown
Confidence level	Medium
Explanation of confidence level	I had a medium sense of confidence on Wolbachia predictions because the line was faint and therefore it was inconclusive.
Wolbachia 16S sequence
Arthropod COI sequence
Summary

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The Wolbachia Project Database