DEM-DCJ Hemiptera

November 11, 2025

Sample information
Picture
Photos by: Devin J.
Location No map location data available.
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected 8/26/25 at 13:20 using sweep net. Habitat of short, fresh cut grass with few trees. Collected in east-westernmost field between Science Center and Jenkins Hall.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Controls worked and all processes went smoothly.
Wolbachia 16S sequence
Arthropod COI sequence
ATAATCATCCGGGTTGAATTAGCACagccattatcgctATTCTTCTCTAA TAACAGACAAGTTTACAATGTAATTATTACTTCTCATGCATTCATTATAA TTTTCTTTATAGTTATACCCATTATAATTGGAGGTTTCGGAAACTGACTT CTCCCTCTTATAATTGGAGCACCTGATATAGCATTTCCTCGTCTTAATAA TATAAGATTCTGACTTCTACCCCCATCATTATCTCTTTTACTTATAAGAT CTATAATTGAAATAGGAGTGGGAACAGGATGAACTGTTTATCCCCCTTTG TCAAGAAACATTGCCCACGCTGGGCCTAGAGTTGACATATCAATTTTTTC ATTACACTTAGCTGGTATCTCATCAATTCTAGGAGCAGTCAATTTTATTA CGACTGTAATAAACATACGACCTACAGGTTTAACACTTCACCAAACACCA TTATTTGTTTGATCTGTTTTAATTACAGCAGTTCTTCTACTATTATCCCT TCCAGTACTTGCAGGGGCAATTACTATACTTTTAACAGACCGAAACATCA ATACAACATTTTTTGACCCCTCAGGAGGGGGTGACCCTATTCTCTATCAA CATTTATTTTGATTTTTTGGTCACC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
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