November 11, 2025

Sample information
Picture
Photos by: Mykhia S.
Location
Collection date 08/25/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

•the traits were:

•stringer

• two sets of wings

•fully developed wings

•Antennas

•Mandibles
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The Wolbachia was not showing in my gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
TGAGCAGGAATAATTGGATCATCAATAAGAATAATTATTCGAATTGAATT AGGATCCTGTGGATCCATTATTAATAATGACCAACTGTATAACTCTATCG TAACAGGACATGCATTTATCATAATTTTCTTTATAGTTATACCATTTATA ATCGGAGGATTTGGTAACTTTTTAATTCCACTAATAATTGGGTCACCAGA TATAGCTTACCCCCGTATAAATAACATAAGATTTTGACTCTTACCCCCAT CAATTATACTACTCACCTTAAGAAGATTTATTAATTCAGGTGCGGGAACT GGTTGAACTGTATACCCTCCCTTATCCTCAAGAATTTTCCATAGGGGAGC CTCAGTAGATTTAGCAATTTTTTCTCTCCATATTGCAGGTATTTCATCAA TCATAGGAGCTATCAACTTCATCTCTACAATTATTAACATAACCCATAAA AATCTTTCAATAGATAAAACCCCTCTCATAGTATGATCTATTCTAATTAC AGCAGTCCTTCTCCTCCTTTCCCTTCCTGTATTAGCCGGAGCAATTACAA TACTACTAACAGATCGAAACCTTAATACTTCATTTTTTGACCCAGCAGGG GGAGGAGATCCTATTCTTTACCAACATTTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
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