Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 08/27/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Unkept Grassy feild with tall grass. sunny |
| Putative identification | Arthropoda Insecta Hemiptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Amplified the extracted insect DNA and then sequenced it with PCR |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GATCCGCAATAAGTGTTATTATTCGAGTAGAATTAGGACaccctgtagag agttATTGGAGATGACCAAATTTATAATGTTATAGTAactgtacATGCAT TTATTATAATTTTCTTTATAGTGATGCCAATTATAATTGGAGGATTTGGA AATTGATTAGTACCTCTAATAATTGGAGCCCCTGATATAGCATTCCCCCG ACTAAATAATATAAGATTTTGATTATTACCCCCCGCTTTAACCTTACTAT TAATGAGAAGATTAACAGAAAGTGGGGCCGGAACTGGATGAACAGTTTAT CCACCTTTATCAAGTAATTTATCCCACAGAGGAGCATCAGTAGATATAGC TATTTTTAGTTTACATTTAGCTGGAGTATCTTCTATTCTAGGAGCAATAA ACTTTATTTCAACTATCATAAATATGCGCCCAGTAGGAATAACACCAGAA CGTATCCCATTATTTGTATGATCAGTTGGAATTACAGCATTACTTTTACT TTTATCCCTACCCGTCTTAGCAGGAGCCATTACAATACTTTtAactGaTc gaAACTTTAATACATCATTCTTCGATCCcttcaggaGGaggggatCCTGT ACTatacccagctttTATttTgatTtTTtggtcACcctgaaaattaacgc ttcttcatactttagtaacataaacaaactcacacttacatttaaattct acacaacagtaatatacctgtttacaccggtaccttatttttccctatcc aacctcctccataccctcacctttctctaaatcacacccaaaagcacgct aacacgaacactccaaaccccctcacttAtccttcttcactacctcaatc tACtCtcgtatctcacaactcttctcagtagacgatatctattttcaaaa aaAAAAAAAAAAAAAAAAAAAAAAAAAAA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Hemiptera was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid