November 12, 2025

Sample information
Picture
Photos by: Tristan M
Location
Collection date 08/27/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Small green insect.
Putative identification Arthropoda Insecta Odonata Coenagrionidae Ischnura Ischnura elegans

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Other
Gel images
Protocol notes

N/A,DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Partner and I were still new to whole process, so there is room for human error.
Wolbachia 16S sequence
GGGGTAAGAGTCAAAATCTTATATTATTTAATCGAGGGaccctatATCTG GTGCCCCTAATATTAGTGGTACTAACCAATTTCCGAACCCACCAATTATG ATTGGTATTACTATAAAAAAAATTATAACAAAAGCGTGTGCAGTTACTAC TACATTATAAATTTGATCATCTCCAATTAGAGATCCTGGTTGTCCGAGTT CAACTCGAATTAATATTCTTAAAGCAGTTCCTACTATACCTGCTCATGCT CCGAACATTAAATATAGGGTTCCAATATCTTTATGattTGTTGAccaaan nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnnnnnnnnnnnnnnnnnnnnnnnnctnnnttttTttttttTTTTTTTT TTTTTTTTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
AGCAGGTATAGTAGGAACTGCTTTAAGAATATTAATTCGAGTTGAACTCG GACAACCagtatcgctAATTGGAGATGATCAAATTTATAATGTAGTAGTA ACttcaCACGCTTTTGTTATAATTTTTTTTATAGTAATACCAATCATAAT TGGTGGGTTCGGAAATTGGTTAGTACCACTAATATTAGGGGCACCAGATA TGGCTTTCCCTCGATTAAATAATATAAGATTTTGACTCTTACCCCCATCT TTAACCCTTCTTCTAGCAAGAAGATTAGTAGAAAGAGGAGCTGGAATTGG TTTCACTcgAAGCTatCCGTtngcngGAGTTATTGCTCATGCTGGAGCGT CAGTAGATTTAACAATTTTTTCATTACATTTAGCGGGAGTATCCTCAATT TTAGGAGCAATTAATTTTATTACAACTACAattnaatATAAAATCACCTG GAATganataTAGATCAATTACCATTGTTTGTATGAGCTGTAGTTATTAC AGCAGTGTTACTTTTATTATCATTACCAGTATTAGCTGGAGCAATTACTA TACTTTTAACTGATCGTAataTTAATACTTCATTTTTTGATCCCGCTGGA GGAGGGGATCCTATCTTATATCAACATTTATTTTGATTTTTTGgtcACCC TgA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Ischnura elegans was found to be postive for Wolbachia.
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