Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/30/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Beat net and gloved hands methodology |
| Putative identification | Arthropoda Insecta Diptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | A Wolbachia-positive DNA sequence for the 16S rRNA gene was not derived |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
CATGAGCAGGAATAGTTGGAACTTCACTAAGTATATTAATTCGAACAGAA TTAAGCCATcccgtttttgttaTTGgngATGATCAAATTTATAATGTAAT TGTtactgtccaTGCTTTTATTATAATTTTTTTTATAGTTATACCAATTA TAATTGGTGGATTTGGAAACTGATTAGTTCCATTAATATTAGGAGCCCCT GATATAGCTTTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCACC TTCTTTATCCCTTCTTCTAATAAGAAGCATAGTTGAAAACGGGGCTGGTA CTGGATGAACAGTATATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGA GCTTCTGTTGATTTAGCAATTTTTTCTCTTCATTTAGCTGGTATTTCTTC TATTTTAGGGGCTGTTAATTTTATTACTACAGTTATTAATATGCGATCTA CTGGAATTACATTTGATCGTATACCACTTTTTGTATGATCTGTAGTTATT ACAGCCGTTTTACTTCTCCTATCCCTCCCAGTTTTAGCCGGGGCTATTAC TATACTATTAACAGATCGAAATTTAAATACAAGTTTCTTTGACCCAGCAG GAGGGGGAGACCCTATTTTATACCAACATTTATTTTGATTTTTTGGTCAC CCTGAAGTT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Diptera was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid