CEM-VEN

November 13, 2025

Sample information
Picture
Photos by: Cassandra M.
Location No map location data available.
Collection date
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Date of collection: 8/28/2025
  • Time of collection: 10:50 am
  • Location of collection: Near Lane Library and Gamble Hall
  • Description of the environment (habitat) of collection site: Caught in bushed, near trees, on the grassy area where there’s sticks, leaves, and other insects
  • Method of collection: Aerial Net, gloves, glass jar, collection tray, and phone to take pictures
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My confidence is medium because I understand what we were doing and did everything correctly, I just kept overthinking about the results and if I did something wrong, even though I didn’t and followed all the steps.
Wolbachia 16S sequence
Arthropod COI sequence
TGGAACATCTTTAAGAATTTTAATTCGAGCTGAATtattacATCCTGGAGCATTAATTGGAGATGATCAAATTTATAATGTAATTGTAACTGCACATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGTGGATTTGGAAATTGATTAGTGCCTTTAATATTAGGTGCTCCTGATATAGCATTCCCACGAATAAATAATATAAGATTTTGACTTCTACCTCCTGCTCTTTCTTTACTATTAGTAAGTAGAATAGTTGAAAATGGAGCTGGGACAGGATGAACTGTTTATCCACCTCTATCCGCTGGAATTGCTCATGGTGGAGCTTCAGTTGATTTAGCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACTGTAATTAATATACGATCAACAGGAATTTCATTAGATCGTATACCTTTATTTGTTTGATCAGTAGTTATTACTGCTTTATTATTATTATTATCACTTCCAGTACTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACATCATTTTTTGACCCAGCGGGAGGAGGAGATCCTATTTTATACCAACATTTATTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be postive for Wolbachia.
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