Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/26/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | NCSSM |
| Observations | Passive Phototactic Sampling using jar collection under existing light. Used existing lights on the outside of our Barn to attract insects. Collected samples using a jar and then soaked arthropods in ethanol. |
| Putative identification | Arthropoda Insecta Lepidoptera Tortricidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Green |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | While we did not get a band for Wolbachia, this is not a definitive answer. This could’ve been due to several reasons and mistakes made during the process. Therefore, I cannot be fully confident there was no Wolbachia in my organism. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
AGTTGGAACTTCCTTAAGATTATTAATTCGAGCTGAATTAGGAAATCCAGGATCATTAATTGGAGATGATCAAATTTATAACACTATTGTTACTGCTCATGCCTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAATTGATTAGTACCTTTAATATTAGGAGCTCCTGATATAGCTTTCCCACGAATAAATAATATAAGATTTTGATTATTACCCCCCTCTCTTATATTATTAATTTCAAGAAGAATTGTAGAAAATGGAGCAGGAACAGGATGAACAGTTTACCCCCCACTTTCATCAAATATTGCTCACAGAGGAAGATCAGTAGATCTTGCTATTTTTTCTCTACATTTAGCTGGAATTTCATCAATTTTAGGAGCTGTTAATTTTATTACAACTATTATTAATATACGACCTAATAATATATCTTTAGATCAAATACCTTTATTTGTTTGATCAGTAGGAATTACAGCTTTATTATTACTTCTTTCTTTACCAGTATTAGCAGGAGCCATTACAATATTATTAACTGATCGAAATCTTAATACATCTTTTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Tortricidae was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid