Sample information

This grasshopper was found in the pollinator garden at NCSSM-Morganton. It was collected at around 1:00 PM using a bug net and identified with the i-Naturalist app.

Methods

DNA Extraction: I removed the majority of the arthropod, other than the abdomen, then took great care while crushing to make sure everything was broken up. 

Gel Lanes:

1. 100bp Ladder
2. Empty
3. American Bird Grasshopper Sample 2 Arthropod Primers
4. American Bird Grasshopper Sample 2 Wolbachia Primers
5. Goldenrod Soldier Beetle Sample 3 Arthropod Primers
6. Goldenrod Soldier Beetle Sample 3 Wolbachia Primers
7. American Bird Grasshopper Sample 3 Arthropod Primers
8. American Bird Grasshopper Sample 3 Wolbachia Primers
9. Empty
10. Arthropod Positive Control
11. Arthropod Negative Control
12. Wolbachia Positive Control
13. Wolbachia Negative Control

Analysis: The controls all worked, and although it was faint, there was a band present for both the arthropod and Wolbachia DNA from the sample.

Results

Our controls all worked and a band was present for the presence of Wolbachia in this sample, but the band was very faint.