Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/26/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | NCSSM |
| Observations | We set up a sheet with an led light behind it, left it for an hour then folded the sheet up and used a jar to capture the bugs. |
| Putative identification | Arthropoda Insecta Coleoptera Chrysomelidae Diabrotica Diabrotica undecimpunctata |
Methods |
|
| Extraction kit | pcr |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | Cyber Green |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | High because we had strong lines across the board and cohesive Wolbachia status results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GGTCAACAAAATCATAAAGATATTGGAACATTATATTTCATTTTTGGAATTTGAGCAGGTATAATTGGAACATCCCTAAGAATTCTAATTCGAGCAGAATTAGGTAGACCCGGTTCCTTAATTGGTAATGATCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATTATAATTGGCGGATTTGGTAACTGACTAGTCCCATTAATAATTGGAGCCCCTGATATAGCATTCCCTCGAATAAATAATATAAGATTTTGACTTTTACCACCCTCTTTATTATTATTATTATTAAGAAGAATAGTAGAAAATGGAGTTGGTACCGGATGAACAGTTTATCCCCCTTTATCATCCAATATTGCTCACGGAGGTTCATCAGTTGATTTAGCTATTTTTAGTTTACACTTGGCTGGAATTTCTTCAATTTTAGGAGCAATTAATTTTATTACTACAGTAATTAATATACGTCCAATAGGAATAACTTTTGACCGAATACCATTATTTGTATGAGCAGTTGTTATTACAGCAGTTTTACTACTATTATCATTACCTGTTCTTGCCGGGGCAATTACAATATTATTAACAGATCGTAATCTAAATACCTCTTTTTTTGATCCTGCTGGAGGTGGTGATCC
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Diabrotica undecimpunctata was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid