Melanoplus bivittatus – Xander Estacio

December 5, 2025

Sample information
Picture
Location
Collection date 09/03/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by fall 2025 section B Lab
Putative identification Arthropoda Insecta Orthoptera Acrididae Melanoplus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

We did not recieve any DNA results, but since around 40% of grasshoppers are projected to have wolbachia it is possibly my insect was infected.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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