7C

February 11, 2026

Sample information
Picture
Entry by: Archer Dougherty
Location
Collection date 09/09/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

I collected this fly in the late afternoon at 4 pm inside the school building. I found it dead on a table; it was a little stuffy, so I would assume it was 70-ish degrees Fahrenheit.
Putative identification Arthropoda Insecta Diptera Dolichopodidae Condylostylus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Condylostylus was found to be negative for Wolbachia.
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