Greenhouse Millipede

January 22, 2026

Sample information
Picture
Entry by: Mae L
Location
Collection date 09/04/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

I found the greenhouse millipede under a potted plant in my front yard. He was found on dirt in a warm environment. He has a mixed complexion of brown and black and many legs. He was found during the fall season in a high 50’s degrees farenheight climate.
Putative identification Arthropoda Diplopoda Polydesmida

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system agarose gel electrophoresis
Buffer TBE
DNA stain
Gel images
Protocol notes

The overall extraction and tests went well; nothing went wrong. My arthropod did not contain wolbachia and I noticed another peer with my same kind of bug did not either, which I thought was interesting.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

In my image, you can clearly see only one presence of DNA in my bug, proving there is no Wolbachia. Another person’s bug in my group tested positive for Wolbachia, which you can clearly see too. Hers is the third gel spot in the plate, and you can see that she has two kinds of DNA showing up, which also shows that my bug does not contain Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Polydesmida was found to be negative for Wolbachia.
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