5O – Black Ant

January 22, 2026

Sample information
Picture
Entry by: Naomi Z
Location
Collection date 09/09/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

I collected it around 10 am, when it was 75(ish) degrees and sunny. It was found walking around on dirt surrounded by lots of plats and trees. It has 6 legs, head, thorax, abdomen, no hair, no wings, and antennae.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

followed Wolbachia project protocols

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Our PCR test was run accordinly and I had lots of DNA collected, making my results most likely accurate
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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