Sample information
Picture	Entry by: Harper L
Location
Collection date	09/08/2025
Captive / Cultivated?	Wild-caught
Group	St. Paul Academy and Summit School
Observations	large greenish brown grasshopper.
Putative identification	Arthropoda Insecta Orthoptera
Methods
Extraction kit	DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location	Abdomen
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	SeeGreen
Gel images
Protocol notes	followed Wolbachia project protocols
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Orthoptera was found to be negative for Wolbachia.