Marsh Crane Fly

January 27, 2026

Sample information
Picture
Entry by: IslaB
Location
Collection date 09/17/2025
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations
Putative identification Arthropoda Insecta Diptera Tipulidae Tipula Tipula oleracea

Methods
Extraction kit Qiagen kit for blood and tissues.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

PCR was not completed accurately for Arthropod, but Wolbachia was done by a different student so students assume the Wolbachia one should be accurate.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Tipula oleracea was found to be negative for Wolbachia.
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