Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/29/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Rutgers Preparatory School |
| Observations | Cloudy, 62 degrees F, 1:16 pm, slight wind. Found on the ground by gazebo. Caught on dirt and grass. |
| Putative identification | Arthropoda Insecta Hymenoptera Vespidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | 1X TAE |
| DNA stain | Ethidium Bromide |
| Gel images |
|
| Protocol notes | Standard protocol |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We successfully got arthropod DNA but the gel clearly showed no Wolbachia either time that we ran it. This brought us to a clear conclusion the arthropod didn’t have a Wolbachia infection. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Vespidae was found to be negative for Wolbachia. |
Lucy B- Arthropod
Spider
Pavement Ant solomon
Soil Centipede