Bee 4

January 14, 2026

Sample information
Picture
Entry by: Irem SUMEN
Location
Collection date 09/25/2025
Captive / Cultivated? Wild-caught
Group Rutgers Preparatory School
Observations

Found in the art room

classification :Yellowjacket and hornets
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain GelGreen
Gel images
Protocol notes

DNA was extracted from the whole bee using the Qiagen DNeasy Blood and Tissue Kit. PCR was performed using arthropod primers (708 bp) and Wolbachia primers (438 bp). Gel electrophoresis with 1X TAE buffer and GelGreen stain confirmed DNA extraction and PCR results. Arthropod DNA was detected, but Wolbachia was not present

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am highly confident in this result because the DNA extraction worked (the 708 bp arthropod band is clearly visible), the PCR reactions were successful as confirmed by positive controls, and the gel staining and electrophoresis worked properly. Since no 438 bp Wolbachia band appeared for Bee 4, we can reliably conclude that this bee is Wolbachia negative
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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