Sample information
Picture	Entry by: Aidan R.
Location	Approximate location: 40.53, -74.49
Collection date	09/25/2025
Captive / Cultivated?	Wild-caught
Group	Rutgers Preparatory School
Observations	Found in a playground, near a gaga pit around 8:30 in the morning.
Putative identification	Arthropoda Insecta Trichoptera
Methods
Extraction kit	Qiagen DNeasy Blood and Tissue Kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Edvotek Gel Electrophoresis
Buffer	TAE
DNA stain	Ethidium Bromide
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Trichoptera was found to be postive for Wolbachia.