Grasshopper 2

January 14, 2026

Sample information
Picture
Entry by: NNC
Location
Collection date 09/25/2025
Captive / Cultivated? Wild-caught
Group Rutgers Preparatory School
Observations

Cloud, 62 degrees F, 1:27 pm, Found in the grass outside, Caught with tweezers.
Putative identification Arthropoda Insecta Orthoptera Gryllidae Velarifictorus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer 1X TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Standard protocol

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

We ran a gel 1 which showed it had both arthropod and Wolbachia DNA and then upon retesting, it was confirmed to be Wolbachia infected.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Velarifictorus was found to be postive for Wolbachia.
Report Inappropriate Post