Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/29/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Rutgers Preparatory School |
| Observations | Cloudy, 62 degrees F, 1:50 pm, Found in the woodchips outside, Caught with the container. |
| Putative identification | Arthropoda Insecta Hemiptera Fulgoridae Lycorma |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | 1X TAE |
| DNA stain | Ethidium Bromide |
| Gel images |
|
| Protocol notes | Standard Protocol |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We received a clear positive Arthropod result and a clear negative Wolbachia result which indicates that the experiment went properly and there is no Wolbachia present. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lycorma was found to be negative for Wolbachia. |



Lucy B- Arthropod
Spider
Pavement Ant solomon
Soil Centipede