Asian Lady Beetle

January 28, 2026

Sample information
Picture
Entry by: Cameron L
Location
Collection date
Captive / Cultivated?
Group Penn State University
Observations

This arthropod was collected from the sidewalk at Penn State University on a fall evening at 5:30 PM. It was about 75 degrees F outside.
Putative identification Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis

Methods
Extraction kit Qiagen DNeasy Blood and Tissue Kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

DNA extraction: the arthropod was crushed, and only the abdomen was removed and dissected.

Gel electrophoresis lanes for CO1 arthropod PCR:

1- DNA Ladder

3- My sample

5- My lab partner’s sample

7- Known arthropod positive for Wolbachia DNA (PCR control)

9- Known arthropod negative for Wolbachia DNA (PCR control)

11- DNA positive control

13- DNA negative control

Analysis: All controls and samples worked properly, showing reliable results.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There were no issues with the protocol, and all controls worked as expected. There were no surprising bands, and for that reason, I am confident that my arthropod was not infected by Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Harmonia axyridis was found to be negative for Wolbachia.
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