Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/20/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Woburn Memorial High School |
| Observations | Small Arthropod Black, almost shriveled Was given to the group for a second test |
| Putative identification | Arthropoda Insecta Isoptera |
Methods |
|
| Extraction kit | Qiagen kit for blood and tissues. |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | 1X TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Figure 1: Gel electrophoresis showing Arthropod DNA results, after 35 minutes of electrophoresis. The 1% agarose gel was stained with 0.5X GelGreen nucleic acid dye with 1X TBE buffer to visualize the DNA bands. Lane 1 contains a 100 bp DNA ladder, lane 2 contains LG arthropod sample (old), lane 3 contains KC arthropod sample (old), lane 4 contains a positive arthropod control (old), lane 5 contains a negative arthropod control (old), lane 6 contains RB arthropod sample (old), lane 7 contains water, lane 8 contains a positive DNA control, lane 9 contains Termite DNA sample (new), lane 10 contains a positive arthropod control (new), and lane 11 contains a negative arthropod control (new) Figure 2: Gel electrophoresis showing Wolbachia DNA results, after 30 minutes after electrophoresis. The 1% agarose gel was stained with 0.5X GelGreen nucleic acid dye with 1X TBE buffer to visualize the DNA bands. Lane 1 contains a 100 bp DNA ladder, lane 2 contains LG wolbachia sample (old), lane 3 contains KC wolbachia sample (old), lane 4 contains a positive wolbachia control (old), lane 5 contains a negative wolbachia control (old), lane 6 contains RB wolbachia sample (old), lane 7 contains water, lane 8 contains a positive DNA control, lane 9 contains Termite DNA sample (new), lane 10 contains a positive wolbachia control (new), and lane 11 contains a negative wolbachia control (new) |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The arthropod gel was not accurate due to poor joy loading the gel. However the Wolbachia gel was accurate, and the results on the termite was accurate. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Isoptera was found to be negative for Wolbachia. |



Pill Bug
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