Fly

March 26, 2026

Sample information
Picture
Entry by: Chloe S
Location
Collection date 12/14/2025
Captive / Cultivated? Wild-caught
Group Coalinga High School
Observations

I found my arthropod on a fly trap in my dining room on the window. The fly was already dead when I collected it and it was fully intact.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

I followed the MiniOne protocol.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My confidence level is low because in both my arthropod and Wolbachia gels, I got a band in my – control and in just my arthropod gel I got a band in my water. This all tells me that my DNA and controls were contaminated and I can not trust the results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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