Sample information

The wasp was frozen on a stucco wall at my school. It was early in the morning.

Methods

DNA Extraction – The abdomen was dissected, but there was an exoskeleton left. The arthropod was not crushed very well.

Gel Electrophoresis Lanes for arthropod.

1- DNA Ladder

2- The second well was a spider.

3-The third well was the wasp sample.

4- unknown

5-unkown

6-unkown

7-unkown

Analysis– In this gel, the wasp did not show lines testing positive for arthropod. There was a problem with DNA extraction. Some exoskeleton remained, and it was not crushed very well. Wells four through seven are labeled unknown because the labels on the tubes got wiped away, and there was no way to know which controls were which. The only labels left on there that were confidently seen were the DNA ladder, the spider, and the wasp.

Gel Electrophoresis Lanes for Wolbachia.

1- DNA Ladder

2- The second well is a spider

3-The third well is the wasp sample

4-Positive arthropod control

5-Positive DNA control

6-Negative arthropod control

7-Water

Analysis– In this gel, there was contamination with well six. The only band shown in this gel was the negative arthropod control. This means the gel results are inconclusive.

Results

The control for PCR did not work as expected in the Wolbachia electrophoresis gel.  There was no band shown in well five, which is the positive DNA control. I was surprised to have a band in the negative arthropod control. This means there was contamination in this control. Therefore, the Wolbachia test is unknown. These results can not be trusted due to the contamination in the negative arthropod control.