Sample information

I found the spider in my room on the floor. I remember it was about 52 degrees that night. The red head on the spider was more vibrant before it was dissolve in alcohol.

Methods

DNA extraction: I don’t think I crushed the abdomen of the parson spider well, because for the Arthropod gel lanes 2-5, there were no bands that showed, which means that there was no DNA detected. For the Wolbachia Gel, it is not guaranteed that the spider was infected with Wolbachia, since there were no results shown in the Arthropod gel.

Gel electrophoresis lanes for arthropod PCR:

1- DNA Ladder

2- Parson spider

3- Roly Poly

4- Positive Arthropod Control

5- Negative Arthropod Control

6- Positive DNA Control

7- Water

Analysis:

Arthropod gel: Lane 6 only showed a band; 2-5 did not show any band at all, which means my group and I made mistakes. We can see that because the controls did not have any bands, which indicates that the experiment did not work or failed.

Wolbachia gel: All the samples did not have any bands. This means that something went wrong with the electrophoresis.

Results

I have low confidence because I can’t draw a conclusion from the result since the controls are inconclusive for both gels, except for lane 6, which was the positive DNA control in Arthropod gel. Therefore, I am not confident whether the spider was infected with Wolbachia or not.