Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Coalinga High School |
| Observations | This spider was found in the garden at around 5 pm while gardening. I flipped a large rock and it was under it as well as some other bugs. |
| Putative identification | Arthropoda Arachnida Araneae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Followed the protocol provided by Mini One. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | I am very confident that we do not know if the arthropod we tested is positively infected with Wolbachia because we did not get any results on our gel for either the arthropod gene or the Wolbachia gene. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Spider
Parson Spider
Wasp
Caterpillar
Fly