Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 12/01/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Coalinga High School |
| Observations | Found in the pot of a tomato plant at roughly 8 pm, kept in a jar for 8 hours, and transferred to a freezer. |
| Putative identification | Arthropoda Insecta Hemiptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Followed Mini One Protocol. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | I am confident that the results for Wolbachia are unknown because the positive control for Wolbachia did not show up on the gel, leading me to believe the results are neither positive nor negative. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Parson Spider
Wasp
Caterpillar
Fruit Fly
Fly