Fruit Fly

Sample information

Picture
Entry by: Analia C
Location
Collection date 11/28/2025
Captive / Cultivated? Wild-caught
Group Coalinga High School
Observations

I found the Fly up on a wall in my restroom. It was hard to capture. I was got it when I had came back from school, and I stepped into my restroom

Putative identification Arthropoda Insecta Diptera

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA Extraction: I tried my best to crush the arthropod well. I believe that due to the size of the  fly, it could’ve been possible that I didn’t crush the arthropod to the best of my ability. I put the whole body of the insect into the vile.

GEL Electrophoresis for CO1 Arthropod DNA

  1. DNA Ladder
  2. The second well is the sample of my insect (Fly)
  3. The third well is the sample of my other insect (Isopod)
  4. The fourth well has my positive Arthropod Control
  5. The fifth well has the negative Arthropod Control
  6. The sixth well has the + DNA Control
  7. The seventh has the water(which shouldn’t appear)

Results

Wolbachia presence No
Confidence level Low
Explanation of confidence level

For the fruit fly, during the Arthropod Gel there wasn’t any visible band in lane 2. There is supposed to be a visible band of your arthropod during the first procedure. As you can see in our first photo, there is no band for the arthropod in Fly lane. In the second electrophoresis, we didn’t get a result for the Fly again.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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