Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/28/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Coalinga High School |
| Observations | I found the Fly up on a wall in my restroom. It was hard to capture. I was got it when I had came back from school, and I stepped into my restroom |
| Putative identification | Arthropoda Insecta Diptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | DNA Extraction: I tried my best to crush the arthropod well. I believe that due to the size of the fly, it could’ve been possible that I didn’t crush the arthropod to the best of my ability. I put the whole body of the insect into the vile. GEL Electrophoresis for CO1 Arthropod DNA
|
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | For the fruit fly, during the Arthropod Gel there wasn’t any visible band in lane 2. There is supposed to be a visible band of your arthropod during the first procedure. As you can see in our first photo, there is no band for the arthropod in Fly lane. In the second electrophoresis, we didn’t get a result for the Fly again. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be negative for Wolbachia. |
Parson Spider
Wasp
Caterpillar
Fruit Fly
Fly