JC2

March 31, 2026

Sample information
Picture
Entry by: Jo'Shanell C
Location
Collection date 03/05/2026
Captive / Cultivated? Wild-caught
Group Auburn University at Montgomery
Observations

Black Ant. Segmented Body, Exoskeleton, Joint Appendages, No Wings
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Cyber Green
Gel images
Protocol notes

Prepared 23 üL PCR cocktail (primers, water, Taq Master Mix) and added 2 üL of JC2 Ant DNA for a total volume of 25 üL.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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