JC1

March 31, 2026

Sample information
Picture
Entry by: Jo'Shanell C
Location
Collection date 03/05/2026
Captive / Cultivated? Wild-caught
Group Auburn University at Montgomery
Observations

Cricket (dark brown & black), hairy with wings
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Prepared 23 üL PCR cocktail ( primers, water, Taq Master Mix) and added 2 üL of JC1 Cricket DNA for a total volume of 25 üL.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Control Worked Correctly:

Positive Control showed bands (PCR Worked )

Negative Control and water: no bands
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be negative for Wolbachia.
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