tenebrio molitor_To1

Sample information

Picture
Location
Collection date 03/06/2026
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

I found it in a forest, some leaves were around it. I collected it in the afternoon, about 2-3 pm.

Putative identification Arthropoda Insecta Coleoptera Tenebrionidae Tenebrio Tenebrio molitor

Methods

Extraction kit Instagene Matrix
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level Low
Explanation of confidence level

There is no band at all, hence maybe the PCR didn’t work.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Tenebrio molitor was found to be negative for Wolbachia.
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