Gesta afranius (Moth)

April 8, 2026

Sample information
Picture
Entry by: Ainsley P
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

It’s brown in color, have wings, there’s tiny furs around
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

High because it has the same location of base pair with the (+) DNA control
Wolbachia 16S sequence
NNNNNGNNTNNNNNNNNNNNNNGTNNNNNANANANNNNNNNNNANNTCTTCNNANNNTTTAATTGNTTAGGTAATCGCTT NCTCTTGATTCGGAGATGATCAAATTTACAGTAGTACTTTTANTNTTCTTGCTTTTATTAGGATTTTNNNTATAGTTATA CCAATTATAATTTTATGATTCTTAGATTGATTAAAACCCCTGATATNAGGAGCTCCTGATATACCTGNTCCCCGAAGAAA TAATATATTATTTTGACTTTTACCCCCCTCTCTAATATTATTAATCTCCAGAAGAATTGTAGAAAATGGAGCTGGAACTG GATGAACTGTTTACCCCCCTCTCTCAGCTAATATTGCACATCAAGGATCATCAGTAGACTTAGCTATTTTTTCCCTTCAT TTAGCTGGAATTTCTTCAATTTTAGGAGCCATTAATTTTATTACAACTATTATTAACATACGAATTAGAAATTTATCATT TGATCAAATACCTTTATTTGTGTGAGCTGTAGGTATTACAGCTTTATTATTATTATTATCATTACCAGTATTAGCTGGGG CCATTACAATACTACTAACTGATCGAAATTTAAATACATCATTTTTTGATCCTGCAGGAGGGGGAGATCCAATTCTTTAC CAACATCTATTTTGATTTTTTGGTCACCCTGAAGTTNAA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
NTNNTNTTNNGGATTTGAGCAGGAATGGTTGGAACTTCTTTAAGTTTATTAATTCGAACTGAATTAGGTAATCCCGGCTC TTTAATTGGAGATGATCAAATTTACAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAA TTATAATTGGAGGATTCGGAAATTGATTAGTACCCCTTATATTAGGAGCTCCTGATATAGCTTTTCCCCGAATAAATAAT ATAAGATTTTGACTTTTACCCCCCTCTCTAATATTATTAATCTCCAGAAGAATTGTAGAAAATGGAGCTGGAACTGGATG AACTGTTTACCCCCCTCTCTCAGCTAATATTGCACATCAAGGATCATCAGTAGACTTAGCTATTTTTTCCCTTCATTTAG CTGGAATTTCTTCAATTTTAGGAGCCATTAATTTTATTACAACTATTATTAACATACGAATTAGAAATTTATCATTTGAT CAAATACCTTTATTTGTGTGAGCTGTAGGTATTACAGCTTTATTATTATTATTATCATTACCAGTATTAGCTGGGGCCAT TACAATACTACTAACTGATCGAAATTTAAATACATCATTTTTTGATCCTGCAGGAGGGGGAGATCCAATTCTTTACCAAC ATCTATTNNNNTTNNNNNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be postive for Wolbachia.
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