Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations | |
| Putative identification | Arthropoda Insecta Hymenoptera Apidae Bombus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | After looking at the gel results, we are positive that we are wolbachia negtive. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
>AFT1-CO1F_C08.ab1 GNNNNGNNAATTCGGATCATCAATAAGATTATTAATTCGAATAGAACTTAGACATCCAGGAATATGAATTAATAATGATC AAATTTATAATTCATTAGTTACTAGACATGCATTTTTAATAATTTTTTTTATAGTTATACCATTTATAATTGGAGGATTT GGAAATTATTTAATTCCTTTAATATTAGGATCACCTGATATAGCTTTTCCACGAATAAATAATATTAGATTTTGATTATT ACCTCCATCTATTTTTATATTATTATTAAGAACTTTATTTACACCTAATGTAGGAACAGGTTGAACCGTATATCCCCCTT TATCATCTTATTTATTTCATTCATCACCTTCAGTAGATATTGCAATTTTTTCTTTACATNTAACAGGAATTTCTTCTATT ATTGGTTCATTAAATTTTATNGNTNCNATTATATTAATAAAAAATTTTTCATTAAATTATGATCAAATTACTTTATTTTC ATGATCTGTATGTATTACAGTNTTATTATTAATTTTATCATTACCAGTTTTAGCNGGAGCAATTACTATACTTCTTTTTG ATCGAAATTTTAATACATCATTTTTTGATCCAATAGGAGGTGGAGATCCAATTTTATATCAACATTTATTTTGANTTTNN NNNCN
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Bombus was found to be negative for Wolbachia. |
Oriental Flower Beetle (Protaetia Orientalis) – Wolbachia Negative
bombus
Spider (Pholcidae)
Pieridea (Sulphurs)