Sample information
Picture	Entry by: Corina B
Location
Collection date
Captive / Cultivated?	Wild-caught
Group	Penn State Lehigh Valley
Observations	Light brown wings with dark brown specks
Putative identification	Arthropoda Insecta
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	1X TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Insecta was found to be negative for Wolbachia.