Sample information
Picture	Entry by: Kalani M
Location
Collection date	09/11/2025
Captive / Cultivated?	Wild-caught
Group	Penn State Lehigh Valley
Observations	Has long legs and a tiny body.
Putative identification	Arthropoda Arachnida Araneae Pholcidae Pholcus Pholcus phalangioides
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	1X TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level	It’s low I had a few mistakes
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Pholcus phalangioides was found to be negative for Wolbachia.