CHI-LYA Orthoptera

April 15, 2026

Sample information
Picture
Location
Collection date 01/28/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Georgia Southern, Armstrong Campus, Arts DR
  • Grassy air that can camouflage itself
  • Hand and jar
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked pretty well while doing the process and everything seemed correct.
Wolbachia 16S sequence
Arthropod COI sequence
AGCAGGTATAGTAGGAACATCAATAAGAATAATTATTCGAGCTGAACTTG GTCAGCCattaTCTCTTATTGGAGATGATCAAATTTATAATGTAATTATT ACAGCACACGCATTTGTAATAATTTTTTTTATAGTTATACCAATTATAAT TGGAGGATTCGGTAATTGATTAGTACCTCTTATAATTGGAGCACCAGACA TAGCCTTTCCTCGAATAAATAATATAAGATTTTGACTATTACCTCCGTCA TTAACCCTTTTATTAATATCCTCTATAGTCGATAATGGAGCTGGTACAGG ATGAACAGTTTACCCACCGTTAGCTAGAACTATTGCCCACAGAGGAGGAG CAGTTGATTTAGCAATTTTTTCTCTACACTTAGCAGGTGTCTCATCTATT CTAGGAGCAGTAAATTTTATTACAACTGCAATTAATATACGATCAGAAAG AATAACATTAGATCAATCACCATTATTTGTTTGATCAGTAGCTATTACAG CATTATTGTTATTACTCTCATTACCAGTTCTAGCAGGTGCCATCACTATA CTATTAACAGACCGAAATCTTAATACATCATTCTTTGATCCAGCTGGAGG AGGTGACCCAATTTTATATCAACACTTATTCTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be negative for Wolbachia.
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