CHI-VR, Hymenoptera

April 15, 2026

Sample information
Picture
Entry by: Valeria R.
Location
Collection date 01/28/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Location of Collection: Found in sunny, dry area behind the GSU Armstrong greenhouses, 8-minute walk from laboratory. Address is 11935 Abercorn St, Savannah, GA 31419. 
  • Habitat of Collection: Found ants in small, domed mound made of soil, pine needles, and twigs. Large amount of nestled twigs above a large portion of the mound.
  • Collection Method: Gloved Hands and Tray
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
  • Rationale:
    • Only 8% of samples for in class  tested for Wolbachia
    • CHIVR’s gel electrophoresis sample did not reflect a Wolbachia 16S gene band 
  • Conclusion:
    • Very low infection probability and my data collection did not support my prediction 
    • CHIVR’s gel electrophoresis sample did not reflect presecnce of Wolbachia 16S gene (lacked 438 bp)
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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