Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 01/22/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Used a sweep net and bare hands for collection by rocks and trees. |
| Putative identification | |
Methods |
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| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | In the gel electrophoresis there was a faint line for Wolbachia presence, but came back with no Wolbachia presence. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTagttg aAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATG CTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTT GGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCC ACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTT TAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTT TACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTT AGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAG TAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTT GATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTT ATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTG ACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCCA ATTTTATACCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
DEM-AES – Hymenoptera
DEM-ORK Order Hymenoptera
(no title)
DEM-OKA Orthoptera
DEM-MGC Coleoptera