DEM-ORK Order Hymenoptera

April 15, 2026

Sample information
Picture
Entry by: Olivia K
Location
Collection date 01/28/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

I used a gloved hands technique, going for ground clutter or debris. I found a large ant hill and used my collection jar to scoop it up. I found my insect in a field/grassland with lots of plants and greenery, close to a pond and a distance away from every day to day human interaction.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Other
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no band for Wolbachia on the electrophoresis results.
Wolbachia 16S sequence
Arthropod COI sequence
ATCATCTATAAGAATAATCATCCGATTAGAACTAGGATctcgtttttcgc taATTAATAATGATCAAATCTACAACTCCTTAGTTACTAGACACGCTTTT ATTATAATTTTTTTTATAGTTATGCCCTTCATAATTGGAGGATTTGGAAA TTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTATCCTCGAA TAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCTTTTACTT ATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTATCTACCC ACCATTAGCCTCCAATATTTTTCACAGAGGAGCCTCTATTGATCTATCTA TTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGCTATTAAT TTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTATAGATAA AATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTTCTTTTAC TTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAACTGACCGT AATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATCCCATTCT ATACCAACATTTATTCTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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