DEM-AES – Hymenoptera

April 15, 2026

Sample information
Picture
Entry by: Adrianna S
Location
Collection date 01/28/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected at 2:00pm. Collected at field and trail behind tennis courts. Environment was dark, damp, and sandy. We looked under leaves, rocks, logs, and at ant hills.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Everything in the gel showed up as it should have. All of the controls were correct, so I trust my results were accurate.
Wolbachia 16S sequence
Arthropod COI sequence
TTGAGCAGGAATAaTTGGTTCATCTATAAGAATAATCATCCGATTAGAAC TAGGATctcgttattgtcTAATTAATAATGATCAAATCTACAACTCCCTA GTTACTAGACACGCTTTTATTATAATTTTTTTTATAGTTATGCCCTTCAT AATTGGGGGATTTGGAAATTTCTTAGTCCCCTTAATACTCGGCTCCCCTG ATATGGCCTATCCTCGAATAAATAATATAAGATTTTGACTTCTACCACCT TCTCTAACTCTTTTACTTATCAGAAGATTTATTAATAGAGGAGTGGGGAC AGGATGAACTATCTATCCACCACTAGCCTCCAATATTTTTCACAGAGGGG CCTCTATTGACCTATCTATTTTTTCCTTACATATCGCCGGAATATCATCA ATCTTAGGGGCTATTAATTTTATTTCTACAATCATTAACATACACCATAA AAATTTTACTATAGATAAAATCCCCCTACTAGTCTGATCTATCCTTATTA CAGCCATTCTTCTTTTACTTTCCCTCCCAGTTCTTGCAGGAGCTATTACA ATATTACTAACTGACCGTAATTTAAACACCTCCTTCTTCGATCCCTCGGG AGGGGGAGATCCCATTCTATATCAACATTTATTCTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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