BRI-DMF Blattodea

April 16, 2026

Sample information
Picture
Entry by: Danielle F
Location
Collection date 01/29/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

 

TIME COLLECTED: 10:00 AM – 11:00 AM
LOCATION OF COLLECTION: The large field located directly east of the Science Center (to the left of the parking lot when facing eastward).
HABITAT: Dormant, dead grasses, arid and dry conditions, many dead sticks and logs
METHOD OF COLLECTION:  We used the beat sticks alternatively to safely flip over heavy dead logs to see insects living underneath.
Putative identification Arthropoda Insecta Blattodea

Methods
Extraction kit pcr
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Our controls worked accurately. We had no major contaminants (ex. RNA).
Wolbachia 16S sequence
GTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCAACTTcct gtatctcGAGTTGCAGAGTACAATCCGAACTGAGATGGCTTTTAAGGGAT TAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACG TGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCT TCCTCCAGTTTATCACTAGCAGTTTCCTTAAAGTCCCCAGCATTACCTGA TGGTAACTAAGGATGAGGGTTACGCTCGTTGCGGGACTTAACCCAACATC TCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAATCCGGCC GAACCGACCCTATCCCTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
ATCATTAAGAATATTAATTCGTGCAGAATTAAATCAAcCttgaTCATTAA TTGGAGATGATCAAATTTATAATGTTATTGTAACAGCTCATGCCTTTGTT ATAATTTTTTTTATAGTTATACCTATTCTTATTGGAGGGTTTGGTAATTG ATTAGTACCTTTAATACTAGGAGCTCCAGATATAGCATTCCCTCGAATAA ATAATATAAGTTTTTGATTACTTCCTCCTTCTTTAACTTTATTATTAGCG AGCAGTATAGTTGAAAGTGGTGCTGGAACAGGTTGAACTGTATACCCACC ACTTGCAAGTGGTATTGCTCATGCAGGAGCTTCTGTAGACTTAGCTATTT TCTCTTTACATTTAGCAGGTGTATCTTCAATTTTGGGAGCAGTTAACTTT ATTTCAACAACAATTAATATAAAACCAATTAATATAAAGCCAGAACGAAT TCCTTTATTTGTTTGAGCTGTAGGAATTACAGCATTATTGTTATTATTAT CATTGCCTGTACTAGCAGGAGCAATTACAATATTACTTACAGATCGTAAT TTAAATACTTCATTTTTTGATCCCGCCGGTGGTGGAGATCCAATTCTTTA TCAACATTTATTCTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattodea was found to be postive for Wolbachia.
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