BRI-FCA Hymenoptera

April 16, 2026

Sample information
Picture
Location
Collection date 01/29/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

On 1/29/2026, 10:00 am, we started to collect specimens near the parking lot in Science Dr using the swoop net. It was very dry and dead.

After 20 minutes of unsuccessful catches, we decided to move northeast to the small lake where there was more greenery and wildlife.

At 10:26 am, we caught our first specimen, a fly! At around 10:30, we found a few ant piles and decided to pick out some ants using soft tweezers.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

According to local data, roughly about 10% of hymenopteras carried Wolbachia. So, I found it highly unlikely that my specimen would have it.
Wolbachia 16S sequence
Arthropod COI sequence
TTGGATCATCTATAAGAATAATCATCCGATTAGAACTAGGATCTTgttat tCTCTAATTAATAATGATCAAATCTACAACTCCTTAGTTACTAGACACGC TTTTAtaaTAATTTTTTTTATAGTTATGCCCTTCATAATTGGAGGATTTG GAAATTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTATCCT CGAATAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCTTTT ACTTATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTATCT ACCCACCATTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGATCTA TCTATTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGCTAT TAATTTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTATAG ATAAAATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTTCTT TTACTTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAACTGA CCGTAATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATCCCA TTCTATACCAACATTTATTCTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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