BRI-KMT Hymenoptera

April 16, 2026

Sample information
Picture
Location
Collection date 01/29/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collection Date – Thursday January 29th 2025 5                                      

Time of Collection – 10:30am 

Location of Collection – outside of GSU Greenhouse

Habitat/Environment – Tall grassy fields and lumps of dirt 

Method of Collection – Sweep Net, Large Specimen Jar
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Lab
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA Template, DNA Polymerase, Primer, Loading Buffer and dNTPs

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

My confidence level was medium because I knew that my insect order typically doesn’t showcase wolbachia but I was hopeful that it did.
Wolbachia 16S sequence
Arthropod COI sequence
TTGGATCATCTATAAGAATAATCATCCGATTAGAACTAGGATCTTgttat tCTCTAATTAATAATGATCAAATCTACAACTCCTTAGTTACTAGACACGC TTTTATTATAATTTTTTTTATAGTTATGCCCTTCATAATTGGAGGATTTG GAAATTTCTTAGTGCCCTTAATACTTGGGTCCCCCGATATGGCCTATCCT CGAATAAATAATATAAGATTTTGACTTCTGCCACCTTCTCTAACTCTTTT ACTTATCAGAAGATTTATTAACAGAGGAGTAGGAACAGGATGAACTATCT ACCCACCATTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGATCTA TCTATTTTTTCCTTACATATCGCCGGAATATCATCAATTTTAGGAGCTAT TAATTTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTATAG ATAAAATCCCCCTACTAGTTTGATCTATCCTTATTACAGCCATTCTTCTT TTACTTTCACTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAACTGA CCGTAATTTAAACACCTCCTTCTTCGATCCCTCAGGAGGAGGAGATCCCA TTCTATACCAACATTTATTCTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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