Butterfly

July 16, 2021

Sample information
Picture
Photos by: Aubrey R.
Location
Collection date 07/07/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

It is a yellow butterfly with brown antennas And brown eyes. It has six legs and a fuzzy body. It was found flying by a river In the shade.
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

The butterfly is in the seventh well. The butterfly’s wings were to big to fit into the tube so I only used the abdomen.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My control didn’t show up.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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