Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/06/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | The Rolly Polly was acting normal in the bushes that are within the school. Nothing out of the normal they were eating and turned into a ball the moment we got close. |
| Putative identification | Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | BioRad |
| Buffer | 1X TAE |
| DNA stain | UV Stain |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | The bands that appeared on the gel were not enough in order to make a good conclusion. Therefore I am sure that the arthropod cannot be talked as having Wolbachia. |
| Wolbachia 16S sequence | Download FASTA
|
| Arthropod COI sequence | Download FASTA
|
| Summary | |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR