Flesh Fly

June 3, 2026

Sample information
Picture
Entry by: Prateek V
Location
Collection date
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations
Putative identification Arthropoda Insecta Diptera Sarcophagidae Sarcophaga Sarcophaga crassipalpis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Arthropod shriveled after being stored in freezer, so dissection took out part of the exoskeleton as well.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

The results were inconclusive for Wolbachia, as there was no positive control band. The results cannot be interpreted as is and the analysis must be redone.

 

This was likely an issue when preparing the samples. This could possibly be an error with the positive control (forgetting the actual positive control DNA) or the error could have propogated through all Wolbachia gel analysis samples (forgetting a common and essential reagent among them all)
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary
Report Inappropriate Post