Brown Crab Spider

Sample information

Picture
Entry by: Ramsey C
Location
Collection date
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations
Putative identification Arthropoda Arachnida Araneae Thomisidae Xysticus

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Body without legs
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results

Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

The gel image is not the most clear. Furthermore, for CO1, the negative drosophila failed. The positive drosophila control for Wolbachia failed as well.

Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary
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